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Fruit and Berries

Fruit and Berries

Analysis of Biomarkers for Cold Tolerance in Strawberry

Biomarkers for cold hardiness are desired for selective breeding of strawberry cultivars for improved winter survival.  Analysis of the crown tissue is favored because it is the most relevant tissue for
overwintering.  We have initially utilized a proteomic approach for biomarker discovery by assessing the
variation of protein profiles from octoploid strawberry cultivars that differ in cold tolerance. Antibodies were obtained for candidate biomarkers and were utilized to validate identification of those candidates. Further analysis of expression of those proteins was performed in both diploid and octoploid
strawberry. We compared the protein expression profiles of the two most
cold-tolerant octoploid cultivars, Senga and Jonsok, with the lesser cold-tolerant cultivars, Frida and Elsanta. Alchohol dehydrogenase, thaumatin, B1-3 glucanase, and dehydrins were chosen for further
analysis.  To investigate the applicability of putative protein markers for predicting cold tolerance, plants derived from crosses of highly Cold tolerant and lesser tolerant octoploid varieties (c.v. ‘Jonsok’ and ‘Elsanta’,
respectively) were evaluated for cold tolerance and expression of several
putative markers. Results indicate significant correlation of several biomarkers
and cold tolerance. The natural variation in cold/freezing tolerance of diploid Fragaria genotypes was
evaluated with expression of candidate markers. Correlation of plant survival with the expression of dehydrins (rdehyd = - 0.82) and alcohol dehydrogenase (radh = - 0.87) in the crown during acclimation indicated that the proteins levels show a clear association with cold tolerance and thus the greatest potential to be developed into biomarkers.  We have identified several of the most Cold responsive dehydrins in the diploid genotypes by purification followed by mass spectroscopy and have analyzed the levels of the protein and corresponding
transcripts during acclimation


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